Individual
DC projects

Research project

Metabolites as epigenetic substrates drive myeloid lineage bias in aging individuals and AML

Rationale and objectives:

Aging skews hematopoiesis toward the myeloid lineage increasing the risk for AML. To the hypothesis that this is caused by age-dependent metabolic changes perturbing epigenetic regulation, we plan:
• To identify changes in the genomic binding of metabolic enzymes associated with age and AML.
• To analyze the levels of related histone PTMs such as lactylation and acylations related with affected metabolic enzymes.
• To investigate the impact of altered histone PTMs on transcription, enhancer activity and chromatin organization.

Envisioned Secondments

RU (Tessarz), months 20-24 (4 months) to receive training in metabolite profiling and acetyl-CoA measurements. P4H (Fernandez), month 27-28 (2 month) to receive training in and perform Seahorse analysis of primary AML cells.

Recruitment

The estimated annual gross salary for the fellow will be €33.000 (approx.).