Individual
DC projects
Research project
Metabolic crosstalk and nuclear acetyl-CoA metabolism in the hematopoietic niche
Rationale and objectives:
Examining the metabolic crosstalk between mesenchymal stem cells (MSC) and leukemic cells and its role in shaping the epigenome is essential to uncover cellular interactions driving leukemia progression.
Therefore, we plan:
• To generate and characterize MSCs and HL-60 AML cells with modulated acetate and acetyl-CoA metabolism.
• To establish co-cultures and measure metabolic flux, metabolic, chromatin and cellular differentiation states in both cell types.
• To identify similar pathways in biopsies and stratify results based on age and leukemic state
Envisioned Secondments
KI (Lennartsson), months 15-19 (4 months) to obtain and characterize bone marrow biopsy material from AML and healthy donors; P4H, months 34-35 (2 months) to receive training in and perform Seahorse analysis in cultured cells.
Recruitment
The estimated annual gross salary for the fellow will be €39.000 (approx.).